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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green Bile Broth
(Brilliant Green Lactose Bile Broth)

培養(yǎng)基配方
Composition per liter:
Oxgall, dehydrated........................................20.0g
Lactose...................................................10.0g
Pancreatic digest of gelatin..............................10.0g
Brilliant Green..........................................0.013g
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to1.0L. Mix thoroughly. Distribute into tubes
containing inverted Durham tubes, in 10.0mL amounts for testing
1.0mL or less of sample. Autoclave for 12 min (not longer than 15 min)
at 15 psi pressure–121°C. After sterilization, cool the broth rapidly.
Medium is sensitive to light.
Use: For the detection of coliform microorganisms in foods, dairy
products, water, and wastewater, as well as in other materials of sani-
tary importance. Turbidity in the broth and gas in the Durham tube are
positive indications of Escherichia coli.