[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-3-9] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromcresol Purple Broth

培養(yǎng)基配方
Composition per liter:
Peptone.....................................................10.0g
NaCl.........................................................5.0g
Beef extract.................................................3.0g
Bromcresol Purple ..........................................0.04g
Carbohydrate solution......................................10.0mL
pH 7.0 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 10.0mL:
Carbohydrate.................................................5.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into test tubes that contain an inverted Durham
tube. Autoclave for 10 min at 15 psi pressure–121°C.
Use: For the differentiation of a variety of microorganisms based on
their fermentation of specific carbohydrates. Bacteria that ferment the
specific carbohydrate turn the medium yellow. When bacteria produce
gas, the gas is trapped in the Durham tube.