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培養(yǎng)基配方
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Dubos Salts Broth with Yeast Extract

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-6-29] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dubos Salts Broth with Yeast Extract

培養(yǎng)基配方啊
Composition per liter:
K2HPO4 ......................................................................1.0g
Yeast extract................................................................0.5g
KCl..........................................................................0.5g
MgSO4 ·7H2O.................................................................0.5g
NaNO3 .......................................................................0.5g
FeSO4 ·7H2O................................................................0.01g
Filter paper strips, sterile ............................................variable
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except filter paper
strips, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Distribute into tubes containing a filter paper strip (filter paper
strip should protrude above the surface of the broth). Autoclave for 15
min at 15 psi pressure–121°C.
Use:  For the cultivation of Cellulomonas species and Cellvibrio spe-
cies.

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