[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-9] [發(fā)布人:] [閱讀次數:] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Esculin Azide HiVeg Broth

培養(yǎng)基配方
Composition per liter:
Plant peptone...............................................................25.0g
Synthetic detergent..........................................................5.0g
Yeast extract................................................................5.0g
Esculin .....................................................................1.0g
Sodium citrate...............................................................1.0g
Ferric ammonium citrate......................................................0.5g
NaN3 .......................................................................0.25g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Cool to 45°–50°C. Do not autoclave.
Use: For the cultivation of entercocci in water, sewage, and feces.