[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-9] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ethanoligenes Medium
(DSMZ Medium 1057)

培養(yǎng)基配方
Composition per liter:
Tryptone ....................................................................4.0g
NaCl.........................................................................4.0g
Beef extract.................................................................2.0g
K2HPO4 ......................................................................1.5g
Yeast extract................................................................1.0g
L -cysteine·HCl ............................................................0.5g
MgCl2 ·6H2O.................................................................0.2g
FeSO4 ·7H2O.................................................................0.1g
Vitamin solution...........................................................10.0mL
Trace elements solution ....................................................1.0mL
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl.............................................................10.0mg
Thiamine-HCl·2H2O..........................................................5.0mg
Riboflavin .................................................................5.0mg
Nicotinic acid..............................................................5.0mg
D -Ca-pantothenate..........................................................5.0mg
p-Aminobenzoic acid.........................................................5.0mg
Lipoic acid ................................................................5.0mg
Biotin .....................................................................2.0mg
Folic acid..................................................................2.0mg
Vitamin B 12 ...............................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 100% N2 . Filter sterilize.
Glucose Solution:
Composition per 10.0mL:
Glucose ....................................................................10.0g
Preparation of Glucose Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge
with 100% N2 . Filter sterilize.
Trace Elements Solution:
Composition per liter:
MgSO4 ·7H2O...............................................................3.0g
Nitrilotriacetic acid......................................................1.5g
NaCl.......................................................................1.0g
MnSO4 ·2H2O...............................................................0.5g
CoSO4 ·7H2O..............................................................0.18g
ZnSO4 ·7H2O..............................................................0.18g
CaCl2 ·2H2O .............................................................0.1g
FeSO4 ·7H2O..............................................................0.1g
NiCl2 ·6H2O............................................................0.025g
KAl(SO42 ·12H2O........................................................0.02g
H3BO3 ..................................................................0.01g
Na2MoO4 ·4H2O..........................................................0.01g
CuSO4 ·5H2O............................................................0.01g
Na2SeO3 ·5H2O..........................................................0.3mg
Preparation of  Trace Elements Solution : Add nitrilotriacetic acid
to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to
6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except glucose and vi-
tamin solutions, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Adjust pH to 6.0. Gently heat while stirring
and bring to boiling. Boil for 1 min. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature while sparging with N2 .
Dispense into tubes or bottles under an atmosphere of 100% N2 . Prior
to inoculation, aseptically and anoxically add the vitamin and glucose
solutions.
Use: For the cultivation of Ethanoligenes spp.