[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-9] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ethyl Violet Azide Broth
(EVA Broth)

培養(yǎng)基配方
Composition per liter:
Pancreatic digest of casein.............................................13.5g
Yeast extract............................................................6.5g
Glucose .................................................................5.0g
NaCl.....................................................................5.0g
K2HPO4 ..................................................................2.7g
KH2PO4 ..................................................................2.7g
NaN3 ....................................................................0.4g
Ethyl Violet...........................................................0.83mg
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the isolation, cultivation, and enumeration of enterococci
from water and other specimens. Fecal enterococci turn the medium
turbid with a purple sediment on the bottom of the tube.