[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-15] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fermentation Medium Base for C. perfringens
with Salicin and Raffinose

培養(yǎng)基配方
Composition per liter:
Casein enzymatic hydrolysate ......................................10.0g
Peptone, special ..................................................10.0g
Agar ...............................................................2.0g
Na-thioglycollate .................................................0.25g
Salicin solution...................................................10.0mL
Raffinose solution.................................................10.0mL
pH 7.0 ± 2.0 at 25°C
Source: This medium, without salicin and raffinose, is available as a
premixed powder from HiMedia.
Salicin Solution:
Composition per 10.0mL:
Salicin..............................................................0.1g
Preparation of Salicin Solution: Add salicin to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Raffinose Solution:
Composition per 10.0mL:
Raffinose...............................................................0.1g
Preparation of Raffinose Solution: Add raffinose to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except salicin and
raffinose solutions, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
10.0mL sterile salicin solution and 10.0mL sterile raffinose solution.
Mix thoroughly. Aseptically distribute into tubes or flasks.
Use:  For the cultivation of Clostridium perfringens.