[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2020-7-15] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ferric Citrate Medium

培養(yǎng)基配方
Composition per liter:
Ferric citrate...............................................................13.7g
Sodium lactate (60% solution).................................................5.6g
NaHCO3 .......................................................................2.5g
NH4Cl ........................................................................1.5g
NaH2PO4 ......................................................................0.6g
KCl...........................................................................0.1g
Wolfe's mineral solution ...................................................10.0mL
Wolfe's vitamin solution ...................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Wolfe's Vitamin Solution:
Composition per liter:
Pyridoxine·HCl.............................................................10.0mg
Calcium  D -(+)-pantothenate.................................................5.0mg
Nicotinic acid...............................................................5.0mg
p-Aminobenzoic acid..........................................................5.0mg
Riboflavin ..................................................................5.0mg
Thiamine·HCl ...............................................................5.0mg
Thioctic acid ...............................................................5.0mg
Biotin ......................................................................2.0mg
Folic acid...................................................................2.0mg
Cyanocobalamin ..............................................................0.1mg
Preparation of Wolfe’s Vitamin Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Wolfe's Mineral Solution:
Composition per liter:
MgSO4 ·7H2O..................................................................3.0g
Nitrilotriacetic acid.........................................................1.5g
NaCl..........................................................................1.0g
MnSO4 ·H2O...................................................................0.5g
CaCl2 ........................................................................0.1g
CoCl2 ·6H2O..................................................................0.1g
FeSO4 ·7H2O .................................................................0.1g
ZnSO4 ·7H2O..................................................................0.1g
A1K(SO4)2 ·12H2O ...........................................................0.01g
CuSO4 ·5H2O.................................................................0.01g
H3BO3 .......................................................................0.01g
Na2MoO4 ·2H2O...............................................................0.01g
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components sequentially. Add distilled/deion-
ized water to 1.0L. Mix thoroughly.
Preparation of Medium: Add ferric citrate to distilled/deionized
water and bring volume to 1.0L. Gently heat and bring to boiling. Con-
tinue boiling until ferric citrate is dissolved. Cool to room temperature.
Adjust to pH 6.6 with 10N NaOH. Add remaining components. Mix
thoroughly. Sparge with 80% N2  + 20% CO2 . Anaerobically distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Fi-
nal pH should be 7.0.
Use: For the cultivation of Aeromonas encheleia and Shewanella alga.