- 定做培養(yǎng)基/定制培養(yǎng)基
- 顆粒培養(yǎng)基
- 標(biāo)準(zhǔn)菌株生化鑒定試劑盒
- 預(yù)灌裝即用型成品培養(yǎng)基
- 2025年版中國(guó)藥典
- 促銷/特價(jià)商品
- 院感/疾控/體外診斷/采樣管
- 樣品采集與處理(均質(zhì))產(chǎn)品
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- 模擬灌裝用培養(yǎng)基
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- 培養(yǎng)基添加劑/補(bǔ)充劑
- 生化反應(yīng)鑒定管
- 染色液等配套產(chǎn)品
- 對(duì)照培養(yǎng)基/標(biāo)準(zhǔn)品
- 實(shí)驗(yàn)耗材與器具
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- 菌種鑒定服務(wù)
Fletcher Medium
山東拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Fletcher Medium
培養(yǎng)基配方
Composition per liter:
Agar ..........................................................................1.5g
NaCl...........................................................................0.5g
Peptone........................................................................0.3g
Beef extract...................................................................0.2g
Rabbit serum.................................................................50.0mL
pH 7.9 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from BD
Diagnostic Systems.
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile
rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the isolation, cultivation, and maintenance of cultures of Lep-
tospira species.