[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2020-7-16] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flexibacter Agar

培養(yǎng)基配方
Composition per liter:
Agar........................................................................15.0g
Monosodium glutamate.........................................................5.0g
Pancreatic digest of casein..................................................1.0g
Vitamin-free casamino acids..................................................1.0g
Sodium glycerophosphate......................................................0.1g
Vitamin B 12 ..............................................................1.0μg
Seawater....................................................................1.0L
Trace elements solution HO-LE..............................................1.0mL
Trace Elements Solution HO-LE:
Composition per liter:
H3BO3 .....................................................................2.85g
MnCl2 ·4H2O ...............................................................1.8g
Sodium tartrate............................................................1.77g
FeSO4 ·7H2O...............................................................1.36g
CoCl2 ·6H2O ..............................................................0.04g
CuCl2· 2H2O..............................................................0.027g
Na2MoO4 ·2H2O ...........................................................0.025g
ZnCl2 ....................................................................0.02g
Preparation of Trace Elements Solution HO-LE: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except traqce elements
solution HO-LE, to filtered seawater and bring volume to 999.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mL of
sterile trace elements solution HO-LE. Mix thoroughly. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Flexibacter polymorphus.