[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-16] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flexibacter Broth

培養(yǎng)基配方
Composition per liter:
Monosodium glutamate......................................................5.0g
Pancreatic digest of casein...............................................1.0g
Vitamin-free casamino acids...............................................1.0g
Sodium glycerophosphate...................................................0.1g
Vitamin B 12 ...........................................................1.0μg
Trace elements solution HO-LE............................................1.0mL
Trace Elements Solution HO-LE:
Composition per liter:
H3BO3 ....................................................................2.85g
MnCl2 ·4H2O ..............................................................1.8g
Sodium tartrate...........................................................1.77g
FeSO4 ·7H2O..............................................................1.36g
CoCl2 ·6H2O..............................................................0.04g
CuCl2· 2H2O.............................................................0.027g
Na2MoO4 ·2H2O...........................................................0.025g
ZnCl2 ....................................................................0.02g
Seawater................................................................999.0mL
Preparation of Trace Elements Solution HO-LE: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except traqce elements
solution HO-LE, to filtered seawater and bring volume to 999.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 1.0mL of sterile trace elements solution
HO-LE. Mix thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use:  For the cultivation of Flexibacter polymorphus.