[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-4-16] [發(fā)布人:李佳頤] [閱讀次數(shù):] [返回]

Hottinger Broth

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Fish peptone................................................................................20.0g
Yeast extract..................................................................................2.0g
Tryptophan....................................................................................1.0g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use:  For cultivation of less fastidious microorganisms and determina-
tion of indole as per USSR State Pharmacopoeia.