[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-4-16] [發(fā)布人:李佳頤] [閱讀次數:] [返回]

Hoyle Medium Base

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Agar............................................................................................15.0g
Beef extract.................................................................................10.0g
Peptone .......................................................................................10.0g
NaCl..............................................................................................5.0g
Blood, laked.............................................................................50.0mL
Tellurite solution......................................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Tellurite Solution:
Composition per 100.0mL:
K 2 TeO 3 ..........................................................................................3.5g
Caution: Potassium tellurite is toxic.
Preparation of Tellurite Solution: Add K 2 TeO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Horse Blood, Laked:
Composition per 50.0mL:
Horse blood, fresh....................................................................50.0mL
Preparation of Horse Blood, Laked: Add blood to a sterile poly-
propylene bottle. Freeze overnight at −20°C. Thaw at 8°C. Refreeze at
−20°C. Thaw again at 8°C.
Preparation of Medium: Add components, except laked blood, to
distilled/deionized water and bring volume to 940.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile laked
blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the isolation and differentiation of Corynebacterium diphthe-
riae.