[所屬分類(lèi):培養(yǎng)基配方] [發(fā)布時(shí)間:2021-4-16] [發(fā)布人:李佳頤] [閱讀次數(shù):] [返回]

Hugh Leifson Glucose Broth

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

NaCl............................................................................................30.0g
Glucose.......................................................................................10.0g
Agar..............................................................................................3.0g

Peptone .........................................................................................2.0g

Yeast extract..................................................................................0.5g

Bromcresol Purple ....................................................................0.015g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Adjust pH to 7.4. Distibute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and differentiation of bacteria based on their
ability to ferment glucose. Bacteria that ferment glucose turn the
medium yellow.