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Persephonella Medium
(DSMZ Medium 996)

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

NaCl ...........................................................................................29.0g
MgSO 4 ·7H 2 O................................................................................7.0g
NaOH ...........................................................................................2.0g
Na 2 S 2 O 3 ........................................................................................2.0g
MgCl 2 ·6H 2 O................................................................................1.36g
KCl ...............................................................................................0.5g
CaCl 2 ·2H 2 O...................................................................................0.4g
K 2 HPO 4 .........................................................................................0.3g
NH 4 Cl ...........................................................................................0.2g
Trace elements solution ..........................................................10.0mL
pH 6.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Na-EDTA·2H 2 O............................................................................0.5g
CoCl 2 ·6H 2 O ................................................................................0.15g
MnCl 2 ·4H 2 O..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
ZnCl 2 .............................................................................................0.1g
AlCl 3 ·6H 2 O..............................................................................40.0mg
Na 2 O 4 W·6H 2 O.........................................................................30.0mg
CuCl.........................................................................................20.0mg
Ni 2 SO 4 ·6H 2 O ...........................................................................20.0mg
Se-acide....................................................................................10.0mg
H 3 BO 3 ......................................................................................10.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................10.0mg
Preparation of Trace Elements Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Adjust pH to 3.0. Mix
thoroughly.
Preparation of Medium: Prepare anaerobic distilled/deionized wa-
ter by sparging with 100% CO 2 . Add components to distilled/deionized
anaerobic water and bring volume to 1.0L. Adjust pH to 6.0 with
H 2 SO 4 . Autoclave for 15 min at 15 psi pressure–121°C. Dispense un-
der a CO 2 atmosphere into Bellco tubes (5mL medium per 27mL tube).
Stopper with butyl stoppers. Cap and crimp closures. Autoclave for 15
min at 15 psi pressure–121°C. After autoclaving a white precipitate
might be present; this precipitate can be redissolved by shaking the me-
dium.It can take up to an hour before all precipitate is dissolved. Add
3.8% O 2 to each tube (1mL of O 2 per 27mL tube). After inoculation
pressurize the tubes with H 2 to 20psi (or 138kPa).
Use:  For the cultivation of Persephonella spp.