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PGT Medium

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Casamino acids...........................................................................30.0g
L- Glutamic acid.............................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.45g
Maltose .........................................................................................0.2g
L- Cystine.......................................................................................0.2g
DL- Tryptophan...............................................................................0.1g
Solution 3...............................................................................100.0mL
Solution 2...................................................................................2.0mL
Calcium pantothenate (0.1% solution) ......................................0.5mL
pH 6.8 ± 0.2 at 25°C
Solution 3:
Composition per 500.0mL:
Maltose .....................................................................................200.0g
CaCl 2 .............................................................................................1.5g
Calcium pantothenate (0.1% solution) ......................................3.0mL
FeSO 4 (1% in 1N HCl) ..............................................................0.2mL
Preparation of Solution 3: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 7 psi pressure–111°C. Cool to 45°–50°C.
Solution 2:
Composition per 100.0mL:
β-Alanine.................................................................................. 0.115g
Nicotinic acid............................................................................ 0.115g
CuSO 4 ·5H 2 O...............................................................................0.05g
ZnSO 4 ·7H 2 O.............................................................................0.045g
MnCl 2 ·4H 2 O .............................................................................0.015g
Pimelic acid ...............................................................................7.5mg
HCl, concentrated......................................................................3.0mL
Preparation of Solution 2: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except solution 3, to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Ad-
just pH to 6.8 with 50% KOH. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
sterile solution 3. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use:  For the cultivation of Corynebacterium diphtheriae.