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PH Medium
(DSMZ Medium 1077)

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per 1118.0mL:

Pancreatic digest of casein............................................................7.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Beef heart, solids from infusion....................................................2.0g
Supplement solution ..............................................................200.0mL
pH 7.8 ± 0.2 at 25°C
Supplement Solution:
Composition per liter:
Horse serum, inactivated........................................................187.0mL
Yeast extract solution...............................................................93.6mL
Fish sperm DNA solution, filter sterilized...............................18.7mL
Yeast Extract Solution:
Composition per 100.0mL:
Yeast extract................................................................................25.0g
Preparation of Yeast Extract Solution : Add yeast extract to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Supplement Solution:  Aseptically add compo-
nents to sterile distilled/deionized water and bring volume to 318.0mL.
Mix thoroughly.
Preparation of Medium:  Add components, except supplement so-
lution, to distilled/deionized water and bring volume to 800.0L. Mix
thoroughly. Adjust pH to 6.5. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically add 318.0mL of supplement solu-
tion. Adjust pH to 7.8. Mix thoroughly. Aseptically distribute into cul-
ture vessels.
Use:  For the cultivation of Mycoplasma orale.