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Phenethyl Alcohol Agar
(Phenylethanol Agar)
(Phenylethyl Alcohol Agar)

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................15.0g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
β-Phenethyl alcohol......................................................................2.5g
Blood........................................................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except blood, to dis-
tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 13 psi pres-
sure–118°C. Cool to 45°–50°C. Aseptically add sterile defibrinated
blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the selective isolation of Gram-positive bacteria, particularly
Gram-positive cocci, from specimens with a mixed flora. Do not use
for the observation of hemolytic reactions.