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Phenol Red Broth

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
NaCl..............................................................................................5.0g
Phenol Red................................................................................0.018g
Carbohydrate solution..............................................................20.0mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Carbohydrate Solution:
Composition per 20.0mL:
Carbohydrate........................................................................5.0–10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 20.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate so-
lution, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Adjust pH to 7.4 if necessary. Distribute into tubes contain-
ing an inverted Durham tube. Fill each tube with 9.8mL of medium. Au-
toclave for 15 min at 13 psi pressure–118°C. Cool to 45°–50°C.
Aseptically add 0.2mL of sterile carbohydrate solution to each tube.
Use: For the determination of fermentation reactions in the differenti-
ation of microorganisms. Fermentation is determined by the produc-
tion of acid—broth turns yellow—and formation of gas—bubble
trapped in Durham tube.