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Phenol Red Glucose Broth
(BAM M122)

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Proteose peptone No. 3...............................................................10.0g
Glucose .........................................................................................5.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................1.0g
Phenol Red solution...................................................................7.2mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostics.
Phenol Red Solution:
Composition per 10.0mL:
Phenol Red................................................................................0.025g
Preparation of Phenol Red Solution: Add Phenol Red to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4 if
necessary. Distribute into tubes containing an inverted Durham tube.
Fill each tube with 10.0mL of medium. Autoclave for 10 min at 13 psi
pressure–118°C.
Use:  For determination of the ability of a microorganism to ferment
glucose. Fermentation is determined by the production of acid—broth
turns yellow—and formation of gas—bubble trapped in Durham tube.