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Phenol Red Glucose Broth with Sodium Chloride
(BAM M122)

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

NaCl............................................................................................30.0g
Proteose peptone No. 3...............................................................10.0g
Glucose.........................................................................................5.0g
Beef extract...................................................................................1.0g
Phenol Red solution...................................................................7.2mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium, without added NaCl, is available as a premixed
powder from BD Diagnostics.
Phenol Red Solution:
Composition per 10.0mL:
Phenol Red................................................................................0.025g
Preparation of Phenol Red Solution: Add Phenol Red to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4 if
necessary. Distribute into tubes containing an inverted Durham tube.
Fill each tube with 10.0mL of medium. Autoclave for 10 min at 13 psi
pressure–118°C.
Use:  For determination of the ability of halophilic Vibrio spp. to fer-
ment glucose. Fermentation is determined by the production of acid—
broth turns yellow—and formation of gas—bubble trapped in Durham
tube.