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Phenol Red Lactose Agar

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Agar............................................................................................15.0g
Lactose........................................................................................10.0g
Proteose peptone No. 3...............................................................10.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................1.0g
Phenol Red...............................................................................25.0mg
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13
psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes. Al-
low tubes to cool in a slanted position.
Use:  For the determination of the ability of a microorganism to ferment
lactose. Fermentation is determined by the production of acid—medium
turns yellow.