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Phenol Red Mannitol Broth

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
D -Mannitol....................................................................................5.0g
NaCl..............................................................................................5.0g
Phenol Red................................................................................0.018g
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3 if
necessary. Distribute into tubes containing an inverted Durham tube.
Fill each tube with 10.0mL of medium. Autoclave for 15 min at 13 psi
pressure–118°C.
Use:  For determination of the ability of a microorganism to ferment
mannitol. Fermentation is determined by the production of acid—broth
turns yellow—and formation of gas—bubble trapped in Durham tube.