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Tindallia Medium (DSMZ Medium 1148)

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-16] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

Tindallia Medium

(DSMZ Medium 1148)

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

NaCl ........................................................................................... 30.0g

Peptone ........................................................................................ 5.0g

NH4Cl .......................................................................................... 1.0g

Yeast extract ................................................................................. 0.5g

KCl ............................................................................................... 0.2g

K2HPO4......................................................................................... 0.2g

MgCl2·6H2O ................................................................................. 0.1g

Resazurin ................................................................................... 1.0mg

Carbonate solution...................................................................10.0mL

Bicarbonate solution ................................................................10.0mL

Sulfide solution........................................................................10.0mL

Vitamin solution.........................................................................2.0mL

Trace elements solution .............................................................1.0mL

pH 9.7 ± 0.2 at 25°C

Sulfide Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................. 0.25g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave

under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room

temperature.

Bicarbonate Solution:

Composition per 10.0mL:

NaHCO3 ........................................................................................ 1.5g

Preparation of Bicarbonate Solution: Add components to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Carbonate Solution:

Composition per 10.0mL:

Na2CO3 ....................................................................................... 2.76g

Preparation of Carbonate Solution: Add components to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Vitamin Solution:

Composition per liter:

Pyridoxine-HCl........................................................................10.0mg

Thiamine-HCl·2H2O..................................................................5.0mg

Riboflavin ..................................................................................5.0mg

Nicotinic acid.............................................................................5.0mg

D-Ca-pantothenate......................................................................5.0mg

p-Aminobenzoic acid.................................................................5.0mg

Lipoic acid .................................................................................5.0mg

Biotin .........................................................................................2.0mg

Folic acid....................................................................................2.0mg

Vitamin B12 ................................................................................0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Sparge

with 80% H2 + 20% CO2. Filter sterilize.

Trace Elements Solution:

Composition per 200.0mL:

MnCl2·4H2O................................................................................ 0.72g

Fe(NH4)2(SO4)2·6H2O ................................................................. 0.4g

FeSO4·7H2O.................................................................................. 0.2g

CoCl2·6H2O .................................................................................. 0.2g

ZnSO4·7H2O ................................................................................. 0.2g

NiCl2·6H2O ................................................................................... 0.1g

Na2MoO4·2H2O ......................................................................... 0.02g

CuSO4·5H2O ............................................................................... 0.02g

H3BO3 ......................................................................................... 0.02g

KAl(SO4)2·12H2O....................................................................... 0.02g

HCl.............................................................................................5.0mL

Preparation of Trace Elements: Add components to distilled/de

ionized water and bring volume to 200.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except sulfide, bicar

bonate, carbonate, and vitamin solutions, to distilled/deionized water

and bring volume to 970.0mL. Mix thoroughly. Sparge with 80% N2 +

20% CO2 gas mixture. Dispense into culture vessels. Autoclave for 15

min at 15 psi pressure–121°C. Cool to room temperature. Aseptically

add sulfide, carbonate, bicarbonate, and vitamin solutions. Mix thor

oughly. Adjust pH to 9.5.–10.0

Use: For the cultivation of Tindallia spp.

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Tindallia Medium (DSMZ Medium 1148)