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Tissue Culture Medium Ham F10 (TC Medium Ham F10)

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-16] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Tissue Culture Medium Ham F10
(TC Medium Ham F10)

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per 1050.0mL:

NaCl.............................................................................................. 7.4g
Glucose ......................................................................................... 1.1g
Na2HPO4..................................................................................... 0.29g
KCl............................................................................................ 0.285g
L-Arginine ................................................................................. 0.211g
MgSO4·7H2O............................................................................ 0.153g
L-Glutamine ............................................................................ 0.1462g
Sodium pyruvate......................................................................... 0.11g
KH2PO4..................................................................................... 0.083g
CaCl2·2H2O .............................................................................. 0.044g
L-Cystine ................................................................................. 0.0315g
L-Lysine................................................................................... 0.0293g
L-Histidine................................................................................. 0.021g
L-Asparagine ............................................................................. 0.015g
L-Glutamic acid....................................................................... 0.0147g
L-Aspartic acid ........................................................................ 0.0133g
L-Leucine ................................................................................ 0.0131g
L-Proline.................................................................................. 0.0115g
L-Serine ................................................................................... 0.0105g
L-Alanine.................................................................................. 8.91mg
Glycine..................................................................................... 7.51mg
L-Phenylalanine........................................................................ 4.96mg
L-Methionine............................................................................ 4.48mg
Hypoxanthine............................................................................. 4.0mg
L-Threonine.............................................................................. 3.57mg
L-Valine ...................................................................................... 3.5mg
L-Isoleucine................................................................................ 2.6mg
L-Tyrosine ................................................................................ 1.81mg
Cyanocobalamin ........................................................................ 1.3mg
Folic acid ................................................................................... 1.3mg
Phenol Red................................................................................. 1.2mg
Thiamine·HCl ............................................................................ 1.0mg
FeSO4·7H2O............................................................................. 0.83mg
Calcium pantothenate ................................................................ 0.7mg
Thymidine.................................................................................. 0.7mg
Choline chloride....................................................................... 0.69mg
Niacinamide............................................................................... 0.6mg
L-Tryptophan.............................................................................. 0.6mg
i-Inositol................................................................................... 0.54mg
Riboflavin ................................................................................ 0.37mg
Lipoic acid ................................................................................. 0.2mg
Pyridoxine·HCl .......................................................................... 0.2mg
ZnSO4·7H2O .......................................................................... 0.028mg
Biotin ..................................................................................... 0.024mg
CuSO4·5H2O...............................................................................2.5μg
Fetal calf serum.............................................................50.0–100.0mL
pH 7.2–7.4 at 25°C
Preparation of Medium: Add components, except fetal calf serum,
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter sterilize. Asep
tically add 50.0–100.0mL of sterile fetal calf serum. Mix thoroughly.
Use: For the cultivation of a wide variety of cell lines in tissue culture.
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