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Todd-Hewitt Broth

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-17] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

Todd-Hewitt Broth

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g

Infusion from 450.0g fat-free minced meat................................ 10.0g

Glucose ......................................................................................... 2.0g

NaHCO3........................................................................................ 2.0g

NaCl.............................................................................................. 2.0g

Na2HPO4....................................................................................... 0.4g

pH 7.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 10 min at 10 psi pressure–115°C.

Use: For the cultivation of group A streptococci used in serological

typing, and for the cultivation of a variety of pathogenic microorgan

isms.

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