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Tomato Juice HiVeg Medium Base with Tomato Juice and Cycloheximide
[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-17] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Tomato Juice HiVeg Medium Base
with Tomato Juice and Cycloheximide
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per liter:
Agar ............................................................................................ 15.0g
Glucose ....................................................................................... 10.0g
Tomato juice solids, from 150.0mL.............................................. 7.5g
Yeast extract.................................................................................. 5.0g
Plant special peptone .................................................................... 5.0g
KH2PO4......................................................................................... 0.5g
CaCl2......................................................................................... 0.125g
MgSO4 ...................................................................................... 0.125g
KCl............................................................................................ 0.125g
NaCl .......................................................................................... 0.125g
Bromcresol Green ....................................................................... 0.03g
MnSO4 ........................................................................................ 0.03g
Tomato juice, canned .............................................................150.0mL
Cycloheximide solution ...........................................................10.0mL
pH 5.0 ± 0.2 at 25°C
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide .............................................................................. 0.1g
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 10.0mL. Mix thorough
ly. Filter sterilize.
Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for
mation and inhalation.
Preparation of Medium: Add components, except cycloheximide
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the isolation and cultivation of lactobacilli.