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培養(yǎng)基配方
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Treponema macrodentium Medium
[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Treponema macrodentium Medium
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per liter:
Glucose ......................................................................................... 1.0g
Nicotinamide................................................................................. 0.4g
Spermine·4HCl ........................................................................... 0.15g
Sodium isobutyrate ..................................................................... 0.02g
Carboxylase ...............................................................................5.0mg
PPLO agar..............................................................................900.0mL
Bovine serum .........................................................................100.0mL
pH 7.0 ± 0.2 at 25°C
PPLO Agar:
Composition per 900.0mL:
Beef heart, infusion from ............................................................ 50.0g
Agar ............................................................................................ 14.0g
Peptone........................................................................................ 10.0g
NaCl .............................................................................................. 5.0g
pH 7.8 ± 0.2 at 25°C
Preparation of PPLO Agar: Add components to distilled/deion
ized water and bring volume to 900.0mL. Mix thoroughly.
Preparation of Medium: Combine components, except bovine se
rum. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add sterile bovine serum. Mix thor
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the isolation and cultivation of Treponema macrodentium.
