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培養(yǎng)基配方
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Treponema Medium
[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Treponema Medium
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per liter:
Spirolate agar.........................................................................675.0mL
Brain heart infusion broth......................................................225.0mL
Rabbit serum,
inactivated at 56°C for 30 min ........................................100.0mL
pH 7.0–7.2 ± 0.2 at 25°C
Spirolate Agar:
Composition per 675.0mL:
Pancreatic digest of casein.......................................................... 15.0g
Ionagar No. 2 ................................................................................ 8.0g
Glucose ......................................................................................... 5.0g
Yeast extract.................................................................................. 5.0g
NaCl.............................................................................................. 2.5g
L-Cysteine·HCl·H2O...................................................................... 1.0g
Sodium thioglycolate.................................................................... 0.5g
Palmitic acid ............................................................................... 0.05g
Stearic acid.................................................................................. 0.05g
Oleic acid.................................................................................... 0.05g
Linoleic acid ............................................................................... 0.05g
Preparation of Spirolate Agar: Add components to distilled/de
ionized water and bring volume to 675.0mL. Mix thoroughly. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Brain Heart Infusion Broth:
Composition per liter:
Pancreatic digest of gelatin......................................................... 14.5g
Brain heart, solids from infusion .................................................. 6.0g
Peptic digest of animal tissue........................................................ 6.0g
NaCl .............................................................................................. 5.0g
Casein............................................................................................ 5.0g
Glucose ......................................................................................... 3.0g
Na2HPO4 ....................................................................................... 2.5g
Preparation of Brain Heart Infusion Broth: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Aseptically combine 675.0mL of cooled,
sterile spirolate agar, 225.0mL of cooled, sterile brain heart infusion
broth, and 100.0mL of rabbit serum. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the isolation of oral treponemes.
