[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2020-7-9] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Esculin Agar, Modified CDC
(BAM M53)

培養(yǎng)基配方
Composition per liter :
Heart muscle, infusion from.................................................375.0g
Agar.........................................................................15.0g
Thiotone.....................................................................10.0g
NaCl..........................................................................5.0g
Esculin ......................................................................1.0g
Ferric citrate................................................................0.5g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Cool to 55°C. Adjust pH to 7.0. Distribute into tubes or
leave in flask. Autoclave for 20 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes. Allow tubes to cool in inclined
position to produce slants.
Use: For the differentiation of Enterobacter spp.