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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ferrous Sulfide Agar

培養(yǎng)基配方
Composition per 1200.0mL:
Agar layer ................................................................1.0L
Liquid overlay..........................................................200.0mL
Agar Layer:
Composition per liter:
Agar ....................................................................30.0g
FeS washed precipitate supension ......................................500.0mL
Preparation of Agar Layer: Add agar to distilled/deionized water
and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Heat FeS washed precipitate suspension to 45°–50°C. Mix thor-
oughly. Aseptically add 500.0mL of sterile FeS washed precipitate supen-
sion to 500.0mL of sterile agar at 45°–50°C. Mix thoroughly.
FeS Washed Precipitate Suspension:
Composition per 500.0mL:
Fe(NH4)2(SO4)2 ·6H2O ..................................................78.4g
Na2S·9H2O..............................................................15.6g
Preparation of FeS Washed Precipitate Suspension: Add
Na2S·9H2O and Fe(NH4)2(SO4)2 ·6H2O to 500.0mL boiling distilled/
deionized water. Let precipitate settle from the hot solution in a com-
pletely filled and stoppered bottle. Wash precipitate four times by de-
canting supernatant and replacing each time with 500.0mL of boiling
distilled/deionized water. Store FeS washed precipitate suspension in a
completely filled 500.0mL glass-stoppered bottle.
Liquid Overlay:
Composition per liter:
(NH4)2Cl ..............................................................1.0g
K2HPO4 ................................................................0.5g
MgSO4 ·7H2O...........................................................0.2g
CaCl2 .................................................................0.1g
Preparation of Liquid Overlay: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically bubble
100% CO2 for 15 sec.
Preparation of Medium: Aseptically distribute agar layer into ster-
ile tubes in 10.0mL volumes. Allow tubes to cool in a slanted poistion.
Aseptically add 2.0mL of sterile liquid overlay to each tube.
Use: For the enumeration, enrichment, and isolation of iron and sulfur
bacteria, including Gallionella ferruginea.