[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-16] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

F-G Broth
(Feeley-Gorman Broth)

培養(yǎng)基配方
Composition per liter:
Casein, acid hydrolyzed...................................................17.5g
Beef extract...............................................................3.0g
Starch.....................................................................1.5g
L -Cysteine solution.....................................................10.0mL
Fe4(P2O7)3 solution......................................................10.0mL
pH 6.9 ± 0.05 at 25°C
L -Cysteine Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H2O......................................................0.4g
Preparation of  L -Cysteine Solution: Add  L- cysteine·HCl·H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Fe4(P2O7)3 Solution:
Composition per 10.0mL:
Fe4(P2O7)3 ...............................................................0.25g
Preparation of Fe4(P2O7)3 Solution: Add Fe4(P2O7)3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except  L -cysteine solu-
tion and Fe4(P2O7)3 solution, to distilled/deionized water and bring vol-
ume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of  L -cysteine solution. Mix thoroughly. Asepti-
cally add 10.0mL of Fe4(P2O7)3 solution. Mix thoroughly. Adjust pH to
6.9. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Legionella pneumophila.