[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-7-16] [發(fā)布人:] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

FGTC Agar

培養(yǎng)基配方
Composition per liter:
Pancreatic digest of casein.................................................15.0g
Agar .......................................................................15.0g
Papaic digest of soybean meal................................................5.0g
NaCl.........................................................................5.0g
KH2PO4 ......................................................................5.0g
Amylose Azure................................................................3.0g
Galactose....................................................................1.0g
Thallous acetate ............................................................0.5g
MUG (4-Methylumbelliferyl-α- D- galactoside.................................0.1g
NaHCO3 solution............................................................20.0mL
Gentamicin solution.........................................................2.5mL
Tween™ 80..................................................................0.75mL
pH 7.3 ± 0.2 at 25°C
Gentamicin Solution:
Composition per 10.0mL:
Gentamicin..................................................................0.01g
Preparation of Gentamicin Solution: Add gentamicin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
NaHCO3 Solution:
Composition per 20.0mL:
NaHCO3 .....................................................................2.0g
Preparation of NaHCO3 Solution: Add the NaHCO3 to distilled/
deionized water and bring volume to 20.0mL. Mix thoroughly. Filter
sterilize. Use freshly prepared solution.
Preparation of Medium: Add components, except NaHCO3 solu-
tion, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°C. Aseptically add sterile NaHCO3
solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation, differentiation, and enumeration of Entero-
coccus species based on starch hydrolysis and production of fluores-
cence. Bacteria that hydrolyze starch, such as Streptococcus bovis,
appear as colonies surrounded by a clear zone. Bacteria that produce
fluorescence, such as Streptococcus bovis and Enterococcus faecium,
appear as colonies surrounded by a zone of bright bluish fluorescence
when viewed under a long-wave UV lamp. Other bacteria, such as
Enterococcus faecalis, Enterococcus avium, or Streptococcus equinus,
do not hydrolyze starch or produce fluorescence.