- 定做培養(yǎng)基/定制培養(yǎng)基
- 顆粒培養(yǎng)基
- 標(biāo)準(zhǔn)菌株生化鑒定試劑盒
- 預(yù)灌裝即用型成品培養(yǎng)基
- 2025年版中國(guó)藥典
- 促銷/特價(jià)商品
- 院感/疾控/體外診斷/采樣管
- 樣品采集與處理(均質(zhì))產(chǎn)品
- 按標(biāo)準(zhǔn)檢索培養(yǎng)基
- 模擬灌裝用培養(yǎng)基
- 干燥粉末培養(yǎng)基
- 培養(yǎng)基添加劑/補(bǔ)充劑
- 生化反應(yīng)鑒定管
- 染色液等配套產(chǎn)品
- 對(duì)照培養(yǎng)基/標(biāo)準(zhǔn)品
- 實(shí)驗(yàn)耗材與器具
- 生化試劑/化學(xué)試劑
- 菌種鑒定服務(wù)
培養(yǎng)基配方
您現(xiàn)在的位置: 網(wǎng)站首頁 >> 培養(yǎng)基配方
Lactic Agar for Yogurt Bacteria, Modified
[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-4-26] [發(fā)布人:李佳頤] [閱讀次數(shù):] [返回]
Lactic Agar for Yogurt Bacteria, Modified
Milk solution............................................................................70.0mL
pH 6.8 ± 0.1 at 25°C
Elliker Agar:
Composition per liter:
Pancreatic digest of casein..........................................................20.0g
Agar ............................................................................................15.0g
Yeast extract................................................................................10.0g
Gelatin...........................................................................................4.0g
Glucose .........................................................................................3.0g
Ascorbic acid ................................................................................2.5g
Lactose..........................................................................................2.5g
NaCl..............................................................................................2.5g
Sodium acetate..............................................................................2.5g
Sucrose..........................................................................................2.5g
Preparation of Elliker Agar: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Milk Solution:
Composition per 100.0mL:
Nonfat dry milk solids ................................................................11.0g
Preparation of Milk Solution: Add nonfat dry milk solids to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 12 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add 70.0mL of sterile milk solution to
1.0L of cooled, sterile Elliker agar. Mix thoroughly. Pour into sterile
Petri dishes. Allow plates to dry at 28°–30°C for 18–24 hr.
Use: For the cultivation of acidogenic microorganisms, especially
Lactobacillus species and lactic streptococci, from foods.
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per liter:
Elliker agar....................................................................................1.0LMilk solution............................................................................70.0mL
pH 6.8 ± 0.1 at 25°C
Elliker Agar:
Composition per liter:
Pancreatic digest of casein..........................................................20.0g
Agar ............................................................................................15.0g
Yeast extract................................................................................10.0g
Gelatin...........................................................................................4.0g
Glucose .........................................................................................3.0g
Ascorbic acid ................................................................................2.5g
Lactose..........................................................................................2.5g
NaCl..............................................................................................2.5g
Sodium acetate..............................................................................2.5g
Sucrose..........................................................................................2.5g
Preparation of Elliker Agar: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Milk Solution:
Composition per 100.0mL:
Nonfat dry milk solids ................................................................11.0g
Preparation of Milk Solution: Add nonfat dry milk solids to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 12 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: Add 70.0mL of sterile milk solution to
1.0L of cooled, sterile Elliker agar. Mix thoroughly. Pour into sterile
Petri dishes. Allow plates to dry at 28°–30°C for 18–24 hr.
Use: For the cultivation of acidogenic microorganisms, especially
Lactobacillus species and lactic streptococci, from foods.