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培養(yǎng)基配方
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Tissue Culture Minimal Medium Eagle
[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-16] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Tissue Culture Minimal Medium Eagle
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per liter:
Sterile salt solution.................................................................944.0mL
TC amino acids, minimal Eagle 50X.......................................20.0mL
TC NaHCO3, 10%....................................................................20.0mL
TC vitamins, minimal Eagle 100X ..........................................10.0mL
TC glutamine, 5% ......................................................................6.0mL
pH 7.2–7.4 at 25°C
Sterile Salt Solution:
Composition per 944.0mL:
NaCl .............................................................................................. 6.8g
Glucose ......................................................................................... 1.0g
KCl................................................................................................ 0.4g
CaCl2............................................................................................. 0.2g
MgCl2............................................................................................ 0.2g
NaH2PO4 ..................................................................................... 0.15g
Preparation of Sterile Salt Solution: Add components to dis
tilled/deionized water and bring volume to 944.0mL. Mix thoroughly.
Filter sterilize.
TC Amino Acids, Minimal Eagle 50X:
Composition per liter:
L-Arginine ..................................................................................... 0.1g
L-Lysine....................................................................................... 0.06g
L-Isoleucine ................................................................................. 0.05g
L-Leucine..................................................................................... 0.05g
L-Threonine ................................................................................. 0.05g
L-Valine ....................................................................................... 0.05g
L-Tyrosine.................................................................................... 0.04g
L-Phenylalanine........................................................................... 0.03g
L-Histidine................................................................................... 0.03g
L-Cystine ..................................................................................... 0.02g
L-Methionine ............................................................................... 0.02g
L-Tryptophan ............................................................................... 0.01g
Preparation of TC Amino Acids, Minimal Eagle 50X: Add
components to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Adjust pH to 7.2–7.4. Filter sterilize.
TC NaHCO3, 10%:
Composition per 100.0mL:
NaHCO3 ...................................................................................... 10.0g
Preparation of TC NaHCO3, 10%: Add NaHCO3 to distilled/de
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
TC Vitamins, Minimal Eagle 100X:
Composition per liter:
Inositol ....................................................................................... 2.0mg
Calcium pantothenate ................................................................ 1.0mg
Choline chloride......................................................................... 1.0mg
Folic acid ................................................................................... 1.0mg
Nicotinamide.............................................................................. 1.0mg
Pyridoxal.................................................................................... 1.0mg
Thiamine·HCl ............................................................................ 1.0mg
Riboflavin .................................................................................. 0.1mg
Preparation of TC Vitamins, Minimal Eagle 100X: Add com
ponents to distilled/deionized water and bring volume to 1.0L. Mix thor
oughly. Filter sterilize.
TC Glutamine, 5%:
Composition per 100.0mL:
L-Glutamine .................................................................................. 5.0g
NaCl (0.85% solution)...........................................................100.0mL
Preparation of TC Glutamine, 5%: Add the glutamine to the
0.85% NaCl solution. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine 944.0mL of sterile
salt solution, 20.0mL of sterile TC amino acids, minimal Eagle 50X,
20.0mL of sterile TC NaHCO3, 10%, 10.0mL of sterile TC vitamins,
minimal Eagle 100X, and 6.0mL of sterile TC glutamine, 5%. Mix
thoroughly. Adjust pH to 7.2–7.4 if necessary.
Use: For the cultivation of mammalian cells in monolayer or suspen
sion for tissue culture procedures and virus preparation.