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培養(yǎng)基配方

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Tissue Culture Minimal Medium Eagle

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-16] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Sterile salt solution.................................................................944.0mL

TC amino acids, minimal Eagle 50X.......................................20.0mL

TC NaHCO3, 10%....................................................................20.0mL

TC vitamins, minimal Eagle 100X ..........................................10.0mL

TC glutamine, 5% ......................................................................6.0mL

pH 7.2–7.4 at 25°C

Sterile Salt Solution:

Composition per 944.0mL:

NaCl .............................................................................................. 6.8g

Glucose ......................................................................................... 1.0g

KCl................................................................................................ 0.4g

CaCl2............................................................................................. 0.2g

MgCl2............................................................................................ 0.2g

NaH2PO4 ..................................................................................... 0.15g

Preparation of Sterile Salt Solution: Add components to dis

tilled/deionized water and bring volume to 944.0mL. Mix thoroughly.

Filter sterilize.

TC Amino Acids, Minimal Eagle 50X:

Composition per liter:

L-Arginine ..................................................................................... 0.1g

L-Lysine....................................................................................... 0.06g

L-Isoleucine ................................................................................. 0.05g

L-Leucine..................................................................................... 0.05g

L-Threonine ................................................................................. 0.05g

L-Valine ....................................................................................... 0.05g

L-Tyrosine.................................................................................... 0.04g

L-Phenylalanine........................................................................... 0.03g

L-Histidine................................................................................... 0.03g

L-Cystine ..................................................................................... 0.02g

L-Methionine ............................................................................... 0.02g

L-Tryptophan ............................................................................... 0.01g

Preparation of TC Amino Acids, Minimal Eagle 50X: Add

components to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Adjust pH to 7.2–7.4. Filter sterilize.

TC NaHCO3, 10%:

Composition per 100.0mL:

NaHCO3 ...................................................................................... 10.0g

Preparation of TC NaHCO3, 10%: Add NaHCO3 to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

TC Vitamins, Minimal Eagle 100X:

Composition per liter:

Inositol ....................................................................................... 2.0mg

Calcium pantothenate ................................................................ 1.0mg

Choline chloride......................................................................... 1.0mg

Folic acid ................................................................................... 1.0mg

Nicotinamide.............................................................................. 1.0mg

Pyridoxal.................................................................................... 1.0mg

Thiamine·HCl ............................................................................ 1.0mg

Riboflavin .................................................................................. 0.1mg

Preparation of TC Vitamins, Minimal Eagle 100X: Add com

ponents to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Filter sterilize.

TC Glutamine, 5%:

Composition per 100.0mL:

L-Glutamine .................................................................................. 5.0g

NaCl (0.85% solution)...........................................................100.0mL

Preparation of TC Glutamine, 5%: Add the glutamine to the

0.85% NaCl solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine 944.0mL of sterile

salt solution, 20.0mL of sterile TC amino acids, minimal Eagle 50X,

20.0mL of sterile TC NaHCO3, 10%, 10.0mL of sterile TC vitamins,

minimal Eagle 100X, and 6.0mL of sterile TC glutamine, 5%. Mix

thoroughly. Adjust pH to 7.2–7.4 if necessary.

Use: For the cultivation of mammalian cells in monolayer or suspen

sion for tissue culture procedures and virus preparation.

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Tissue Culture Minimal Medium Eagle