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Tissue Culture Minimal Medium Eagle with Earle Balanced Salts Solution (TC Minimal Medium Eagle with Earle BSS)

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-16] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Tissue Culture Minimal Medium Eagle
with Earle Balanced Salts Solution
(TC Minimal Medium Eagle with Earle BSS)

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per 1056.0mL:

NaCl.............................................................................................. 6.8g
Glucose ......................................................................................... 1.0g
KCl................................................................................................ 0.4g
CaCl2·2H2O .................................................................................. 0.2g
MgCl2·6H2O ................................................................................. 0.2g
NaH2PO4..................................................................................... 0.15g
L-Arginine ..................................................................................... 0.1g
L-Lysine....................................................................................... 0.06g
L-Isoleucine................................................................................. 0.05g
L-Leucine .................................................................................... 0.05g
L-Threonine................................................................................. 0.05g
L-Valine ....................................................................................... 0.05g
L-Tyrosine ................................................................................... 0.04g
L-Phenylalanine........................................................................... 0.03g
L-Histidine................................................................................... 0.03g
L-Cystine ..................................................................................... 0.02g
L-Methionine............................................................................... 0.02g
L-Tryptophan............................................................................... 0.01g
i-Inositol..................................................................................... 2.0mg
Calcium pantothenate ................................................................ 1.0mg
Choline chloride......................................................................... 1.0mg
Folic acid ................................................................................... 1.0mg
Nicotinamide.............................................................................. 1.0mg
Pyridoxal.................................................................................... 1.0mg
Thiamine·HCl ............................................................................ 1.0mg
Riboflavin .................................................................................. 0.1mg
Serum............................................................................50.0–100.0mL
Glutamine solution.....................................................................6.0mL
CaCl2·2H2O solution (optional).................................................2.0mL
pH 7.2–7.4 at 25°C
Glutamine Solution:
Composition per 100.0mL:
L-Glutamine................................................................................... 5.0g
NaCl (0.85% solution) ...........................................................100.0mL
Preparation of Glutamine Solution: Add the glutamine to the
0.85% NaCl solution. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except glutamine and
serum, to distilled/deionized water and bring volume to 1.0L. Mix thor
oughly. Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter steril
ize. Aseptically add 6.0mL of sterile glutamine solution and 50.0–
100.0mL of sterile serum. Human serum, bovine serum, horse serum,
or fetal calf serum may be used. Mix thoroughly. To grow cells in a
monolayer, aseptically add 2.0mL of a sterile 10% CaCl2·2H2O solu
tion. To grow cells in suspension, omit the CaCl2·2H2O solution.
Use: For preparation of Eagle’s minimal medium for the cultivation of
cells in monolayer or suspension in tissue culture.
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