展開

編號：
批號：

培養(yǎng)基配方

您現(xiàn)在的位置：網(wǎng)站首頁 >> 培養(yǎng)基配方

Tissue Culture Minimal Medium Eagle with Earle Balanced Salts Solution (TC Minimal Medium Eagle with Earle BSS)

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-16] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

Tissue Culture Minimal Medium Eagle

with Earle Balanced Salts Solution

(TC Minimal Medium Eagle with Earle BSS)

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per 1056.0mL:

NaCl.............................................................................................. 6.8g

Glucose ......................................................................................... 1.0g

KCl................................................................................................ 0.4g

CaCl2·2H2O .................................................................................. 0.2g

MgCl2·6H2O ................................................................................. 0.2g

NaH2PO4..................................................................................... 0.15g

L-Arginine ..................................................................................... 0.1g

L-Lysine....................................................................................... 0.06g

L-Isoleucine................................................................................. 0.05g

L-Leucine .................................................................................... 0.05g

L-Threonine................................................................................. 0.05g

L-Valine ....................................................................................... 0.05g

L-Tyrosine ................................................................................... 0.04g

L-Phenylalanine........................................................................... 0.03g

L-Histidine................................................................................... 0.03g

L-Cystine ..................................................................................... 0.02g

L-Methionine............................................................................... 0.02g

L-Tryptophan............................................................................... 0.01g

i-Inositol..................................................................................... 2.0mg

Calcium pantothenate ................................................................ 1.0mg

Choline chloride......................................................................... 1.0mg

Folic acid ................................................................................... 1.0mg

Nicotinamide.............................................................................. 1.0mg

Pyridoxal.................................................................................... 1.0mg

Thiamine·HCl ............................................................................ 1.0mg

Riboflavin .................................................................................. 0.1mg

Serum............................................................................50.0–100.0mL

Glutamine solution.....................................................................6.0mL

CaCl2·2H2O solution (optional).................................................2.0mL

pH 7.2–7.4 at 25°C

Glutamine Solution:

Composition per 100.0mL:

L-Glutamine................................................................................... 5.0g

NaCl (0.85% solution) ...........................................................100.0mL

Preparation of Glutamine Solution: Add the glutamine to the

0.85% NaCl solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glutamine and

serum, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter steril

ize. Aseptically add 6.0mL of sterile glutamine solution and 50.0–

100.0mL of sterile serum. Human serum, bovine serum, horse serum,

or fetal calf serum may be used. Mix thoroughly. To grow cells in a

monolayer, aseptically add 2.0mL of a sterile 10% CaCl2·2H2O solu

tion. To grow cells in suspension, omit the CaCl2·2H2O solution.

Use: For preparation of Eagle’s minimal medium for the cultivation of

cells in monolayer or suspension in tissue culture.

返回

購物車去頂部

免费国产直接看片_日韩三级精品一区_久久久黄色视频深圳大学_久久久久成亚洲综合精品_久久久av一区二区三_国产AV成人无码精品网站_色愉拍亚洲偷自拍_亚洲欧美另类激情综合区183_欧美成人午夜免费_国产AV无码片毛片一久

在線客服

Tissue Culture Minimal Medium Eagle with Earle Balanced Salts Solution (TC Minimal Medium Eagle with Earle BSS)