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培養(yǎng)基配方

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Tissue Culture Minimal Medium Eagle Spinner Modified (TC Minimal Medium Eagle Spinner Modified MEM-S)

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-16] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

Tissue Culture Minimal Medium

Eagle Spinner Modified

(TC Minimal Medium Eagle Spinner Modified MEM-S)

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per 1056.0mL:

NaH2PO4 ..................................................................................... 1.35g

NaCl .............................................................................................. 6.8g

NaHCO3 ........................................................................................ 2.2g

Glucose ......................................................................................... 1.0g

KCl................................................................................................ 0.4g

CaCl2·2H2O................................................................................... 0.2g

NaH2PO4 ................................................................................... 0.125g

MgSO4·7H2O ................................................................................ 0.1g

L-Isoleucine ............................................................................... 0.026g

L-Leucine................................................................................... 0.026g

L-Lysine..................................................................................... 0.026g

L-Threonine ............................................................................... 0.024g

L-Valine ................................................................................... 0.0235g

L-Tyrosine.................................................................................. 0.018g

L-Arginine ............................................................................... 0.0174g

L-Phenylalanine....................................................................... 0.0165g

L-Cystine ................................................................................... 0.012g

L-Histidine..................................................................................8.0mg

L-Methionine ..............................................................................7.5mg

Phenol Red.................................................................................5.0mg

L-Tryptophan..............................................................................4.0mg

Inositol .......................................................................................1.8mg

Biotin .........................................................................................1.0mg

Calcium pantothenate ................................................................1.0mg

Choline chloride.........................................................................1.0mg

Folic acid....................................................................................1.0mg

Nicotinamide..............................................................................1.0mg

Pyridoxal·HCl ............................................................................1.0mg

Thiamine·HCl ............................................................................1.0mg

Riboflavin ..................................................................................0.1mg

Serum ......................................................................50.0mL–100.0mL

Glutamine solution.....................................................................6.0mL

pH 7.2–7.4 at 25°C

Glutamine Solution:

Composition per 100.0mL:

L-Glutamine .................................................................................. 5.0g

NaCl (0.85% solution)...........................................................100.0mL

Preparation of Glutamine Solution: Add the glutamine to the

0.85% NaCl solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glutamine and

serum, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter steril

ize. Aseptically add 6.0mL of sterile glutamine solution and 50.0–

100.0mL of sterile serum. Human serum, bovine serum, horse serum,

or fetal calf serum may be used. Mix thoroughly.

Use: For the cultivation of mammalian cells in suspension.

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Tissue Culture Minimal Medium Eagle Spinner Modified (TC Minimal Medium Eagle Spinner Modified MEM-S)