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Tissue Culture Minimal Medium Eagle Spinner Modified (TC Minimal Medium Eagle Spinner Modified MEM-S)

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-16] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Tissue Culture Minimal Medium
Eagle Spinner Modified
(TC Minimal Medium Eagle Spinner Modified MEM-S)

山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per 1056.0mL:

NaH2PO4 ..................................................................................... 1.35g
NaCl .............................................................................................. 6.8g
NaHCO3 ........................................................................................ 2.2g
Glucose ......................................................................................... 1.0g
KCl................................................................................................ 0.4g
CaCl2·2H2O................................................................................... 0.2g
NaH2PO4 ................................................................................... 0.125g
MgSO4·7H2O ................................................................................ 0.1g
L-Isoleucine ............................................................................... 0.026g
L-Leucine................................................................................... 0.026g
L-Lysine..................................................................................... 0.026g
L-Threonine ............................................................................... 0.024g
L-Valine ................................................................................... 0.0235g
L-Tyrosine.................................................................................. 0.018g
L-Arginine ............................................................................... 0.0174g
L-Phenylalanine....................................................................... 0.0165g
L-Cystine ................................................................................... 0.012g
L-Histidine..................................................................................8.0mg
L-Methionine ..............................................................................7.5mg
Phenol Red.................................................................................5.0mg
L-Tryptophan..............................................................................4.0mg
Inositol .......................................................................................1.8mg
Biotin .........................................................................................1.0mg
Calcium pantothenate ................................................................1.0mg
Choline chloride.........................................................................1.0mg
Folic acid....................................................................................1.0mg
Nicotinamide..............................................................................1.0mg
Pyridoxal·HCl ............................................................................1.0mg
Thiamine·HCl ............................................................................1.0mg
Riboflavin ..................................................................................0.1mg
Serum ......................................................................50.0mL–100.0mL
Glutamine solution.....................................................................6.0mL
pH 7.2–7.4 at 25°C
Glutamine Solution:
Composition per 100.0mL:
L-Glutamine .................................................................................. 5.0g
NaCl (0.85% solution)...........................................................100.0mL
Preparation of Glutamine Solution: Add the glutamine to the
0.85% NaCl solution. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except glutamine and
serum, to distilled/deionized water and bring volume to 1.0L. Mix thor
oughly. Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter steril
ize. Aseptically add 6.0mL of sterile glutamine solution and 50.0–
100.0mL of sterile serum. Human serum, bovine serum, horse serum,
or fetal calf serum may be used. Mix thoroughly.
Use: For the cultivation of mammalian cells in suspension.
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