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Toluidine Blue DNA Agar

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-17] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Agar ............................................................................................ 10.0g

NaCl............................................................................................ 10.0g

Tris(hydroxymethyl)aminomethane buffer................................... 6.1g

Deoxyribonucleic acid.................................................................. 0.3g

Toluidine Blue O....................................................................... 0.083g

CaCl2, anhydrous....................................................................... 1.1mg

pH 9.0 ± 0.2 at 25°C

Preparation of Medium: Add tris(hydroxymethyl)aminomethane

buffer to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Adjust pH to 9.0. Add the remaining components, except Tol

uidine Blue O. Mix thoroughly. Gently heat and bring to boiling. Add

Toluidine Blue O. Mix thoroughly. If used the same day, sterilization is

not necessary. Cool to 50°C. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation and differentiation of Staphylococcus aureus

from foods.

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