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Toluidine Blue DNA Agar

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-17] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Agar ............................................................................................ 10.0g

NaCl............................................................................................ 10.0g

Tris(hydroxymethyl)aminomethane buffer................................... 6.1g

Deoxyribonucleic acid (DNA)...................................................... 0.3g

Toluidine Blue O....................................................................... 0.083g

CaCl2, anhydrous....................................................................... 1.1mg

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except Toluidine Blue

O, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Add Toluidine Blue O. Mix

thoroughly. Medium does not have to be sterilized if used immediately.

Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes

to cool in a slanted position.

Use: For the cultivation and differentiation of bacteria based on their

production of deoxyribonuclease (DNase). Bacteria that produce DNase

turn the medium pink.

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