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培養(yǎng)基配方

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Transgrow Medium

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

Transgrow Medium

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

GC medium base....................................................................730.0mL

Hemoglobin solution..............................................................250.0mL

Supplement B...........................................................................10.0mL

VCNT antibiotic solution.........................................................10.0mL

pH 7.3 ± 0.2 at 25°C

GC Medium Base:

Composition per 730.0mL:

Proteose peptone No. 3 ............................................................... 15.0g

Agar ............................................................................................ 20.0g

NaCl .............................................................................................. 5.0g

K2HPO4......................................................................................... 4.0g

Glucose ......................................................................................... 1.5g

Cornstarch..................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

pH 7.2 ± 0.2 at 25°C

Preparation of GC Medium Base: Add components to distilled/

deionized water and bring volume to 730.0mL. Mix thoroughly. Gently

heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C.

Hemoglobin Solution:

Composition per 250.0mL:

Hemoglobin ................................................................................ 10.0g

Preparation of Hemoglobin Solution: Add hemoglobin to dis

tilled/deionized water and bring volume to 250.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Supplement B:

Composition per 10.0mL:

Supplement B contains yeast concentrate, glutamine, coenzyme, co

carboxylase, hematin, and growth factors.

Preparation of Supplement B: Add components to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Source: Supplement B is available as a premixed powder from BD

Diagnostic Systems.

VCNT Antibiotic Solution:

Composition per 10.0mL:

Colistin methane sulfonate.........................................................7.5mg

Trimethoprim lactate..................................................................5.0mg

Vancomycin ...............................................................................3.0mg

Nystatin.................................................................................. 12,500U

Preparation of VCNT Antibiotic Solution: Add components to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: To 730.0mL of cooled, sterile GC medi

um base, aseptically add 250.0mL of sterile hemoglobin solution,

10.0mL of sterile supplement B, and 10.0mL of VCNT antibiotic solu

tion. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation and transport of fastidious microorganisms,

especially Neisseria species.

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Transgrow Medium