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培養(yǎng)基配方
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Transgrow Medium with Trimethoprim
[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Transgrow Medium with Trimethoprim
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per liter:
Agar ............................................................................................ 20.0g
Hemoglobin ................................................................................ 10.0g
Pancreatic digest of casein............................................................ 7.5g
Selected meat peptone .................................................................. 7.5g
NaCl.............................................................................................. 5.0g
K2HPO4......................................................................................... 4.0g
Glucose ......................................................................................... 1.5g
Cornstarch..................................................................................... 1.0g
KH2PO4......................................................................................... 1.0g
Supplement solution ................................................................10.0mL
VCNT inhibitor........................................................................10.0mL
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di
agnostic Systems.
Supplement Solution:
Composition per liter:
Glucose ..................................................................................... 100.0g
L-Cysteine·HCl............................................................................ 25.9g
L-Glutamine ................................................................................ 10.0g
L-Cystine ....................................................................................... 1.1g
Adenine......................................................................................... 1.0g
Nicotinamide adenine dinucleotide ............................................ 0.25g
Vitamin B12 ................................................................................... 0.1g
Thiamine pyrophosphate .............................................................. 0.1g
Guanine·HCl ............................................................................... 0.03g
Fe(NO3)3·6H2O........................................................................... 0.02g
p-Aminobenzoic acid................................................................ 0.013g
Thiamine·HCl ............................................................................ 3.0mg
Source: The supplement solution (IsoVitaleX® enrichment) is avail
able from BD Diagnostic Systems. This enrichment may be replaced
by supplement VX from BD Diagnostic Systems.
Preparation of Supplement Solution: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
VCNT Inhibitor:
Composition per 10.0mL:
Colistin....................................................................................... 7.5mg
Trimethoprim lactate.................................................................. 5.0mg
Vancomycin ............................................................................... 3.0mg
Nystatin.................................................................................. 12,500U
Preparation of VCNT Inhibitor: Add components to distilled/de
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster
ilize.
Preparation of Medium: Add components, except supplement so
lution and VCNT inhibitor, to distilled/deionized water and bring vol-
ume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C un
der 5–30% CO2. Aseptically add 10.0mL of sterile supplement solu
tion and 10.0mL of sterile VCNT inhibitor. Mix thoroughly.
Aseptically distribute under 5–30% CO2 into sterile screw-capped
tubes.
Use: For the transportation and recovery of pathogenic Neisseria spe
cies.
