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Treponema denticola Medium (DSMZ Medium 909)

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

Treponema denticola Medium

(DSMZ Medium 909)

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per 1045.0mL:

Solution A.....................................................................................1.0L

Solution B ................................................................................45.0mL

pH 7.1 ± 0.2 at 25°C

Solution A:

Composition per liter:

Trypticase™................................................................................ 10.0g

Yeast extract.................................................................................. 2.5g

Agar .............................................................................................. 3.0g

Glucose ......................................................................................... 2.0g

L-Cysteine·HCl ............................................................................. 1.0g

Na-thioglycolate ........................................................................... 0.5g

L-Asparagine............................................................................... 0.25g

Brain heart infusion broth......................................................450.0mL

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Boil for 5 min. Cool to room temperature while sparging

with 80% N2 + 20% CO2. Distribute to anaerobe tubes or bottles under

80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to room temperature.

Brain Heart Infusion Broth:

Composition per liter:

Pancreatic digest of gelatin ......................................................... 14.5g

Brain heart, solids from infusion .................................................. 6.0g

Peptic digest of animal tissue........................................................ 6.0g

NaCl .............................................................................................. 5.0g

Casein............................................................................................ 5.0g

Glucose ......................................................................................... 3.0g

Na2HPO4 ....................................................................................... 2.5g

Preparation of Brain Heart Infusion Broth: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Solution B:

Composition per 65.0mL:

NaHCO3 ........................................................................................ 2.0g

Thiamine pyrophosphate............................................................6.0mg

Volatile fatty acid solution .......................................................20.0mL

Rabbit serum ............................................................................20.0mL

Volatile Fatty Acid Solution:

Composition per 102.0mL:

KOH, 0.1N.............................................................................100.0mL

Isobutyric acid............................................................................0.5mL

D,L-2-methylbutyric acid ...........................................................0.5mL

Isovaleric acid ............................................................................0.5mL

Valeric acid ................................................................................0.5mL

Preparation of Volatile Fatty Acid Solution: Combine compo

nents and mix thoroughly.

Preparation of Solution B: Add components, except rabbit serum,

to distilled/deionized water and bring volume to 45.0mL. Mix thor

oughly. Filter sterilize. Add 20.0mL rabbit serum.

Preparation of Medium: Aseptically and anaerobically add

0.45mL solution B and 10.0mL solution A to individual tubes. Pressur

ize the tubes with H2 to 0.5 bar.

Use: For the cultivation of Treponema denticola.

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Treponema denticola Medium (DSMZ Medium 909)