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培養(yǎng)基配方
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Treponema Isolation Medium
[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Treponema Isolation Medium
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per liter:
Solution A ..............................................................................450.0mL
Spirolate broth........................................................................450.0mL
Rabbit serum,
inactivated at 56°C for 30 min ........................................100.0mL
pH 7.4 ± 0.2 at 25°C
Solution A:
Composition per 450.0mL:
Agar .............................................................................................. 8.0g
Asparagine .................................................................................. 0.25g
Sodium thioglycolate .................................................................. 0.25g
Pancreatic digest of casein.......................................................... 0.25g
Brain heart infusion broth ......................................................450.0mL
Preparation of Solution A: Combine components. Mix thorough
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C.
Brain Heart Infusion Broth:
Composition per liter:
Pancreatic digest of gelatin......................................................... 14.5g
Brain heart, solids from infusion .................................................. 6.0g
Peptic digest of animal tissue ....................................................... 6.0g
NaCl.............................................................................................. 5.0g
Casein ........................................................................................... 5.0g
Glucose ......................................................................................... 3.0g
Na2HPO4....................................................................................... 2.5g
Preparation of Brain Heart Infusion Broth: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Spirolate Broth:
Composition per liter:
Pancreatic digest of casein.......................................................... 15.0g
Glucose ......................................................................................... 5.0g
Yeast extract.................................................................................. 5.0g
NaCl.............................................................................................. 2.5g
L-Cysteine·HCl·H2O ..................................................................... 1.0g
Sodium thioglycolate.................................................................... 0.5g
Palmitic acid ............................................................................... 0.05g
Stearic acid.................................................................................. 0.05g
Oleic acid.................................................................................... 0.05g
Linoleic acid ............................................................................... 0.05g
Preparation of Spirolate Broth: Add components to distilled/de
ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Combine 450.0mL of sterile solution A,
450.0mL of sterile spirolate broth, and 100.0mL of rabbit serum. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the isolation and cultivation of oral, genital, and fecal
treponemes.
