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Treponema Medium

[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

Treponema Medium

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Spirolate agar.........................................................................900.0mL

Rabbit serum,

inactivated at 56°C for 30 min ........................................100.0mL

Spirolate Agar:

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Agar ............................................................................................ 14.0g

Glucose ......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NaCl.............................................................................................. 2.5g

L-Cysteine·HCl·H2O...................................................................... 1.0g

Sodium thioglycolate.................................................................... 0.5g

Palmitic acid ............................................................................... 0.05g

Stearic acid.................................................................................. 0.05g

Oleic acid.................................................................................... 0.05g

Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Agar: Add components to distilled/de

ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C.

Preparation of Medium: To 900.0mL of cooled, sterile spirolate

agar, aseptically add 100.0mL of rabbit serum. Mix thoroughly. Asep

tically distribute into sterile tubes or flasks.

Use: For the isolation of oral treponemes.

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