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培養(yǎng)基配方

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Treponema Medium

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

Treponema Medium

山東拓普生物工程有限公司培養(yǎng)基配方 http://www.jlkcpj.cn

Composition per liter:

Solution A ..............................................................................440.0mL

Spirolate broth........................................................................440.0mL

Rabbit serum,

inactivated at 56°C for 30 min ........................................100.0mL

Mucin solution .........................................................................20.0mL

pH 7.8 ± 0.2 at 25°C

Solution A:

Composition per 440.0mL:

Ionagar No. 2 ................................................................................ 8.0g

Brain heart infusion broth ......................................................440.0mL

Brain Heart Infusion Broth:

Composition per liter:

Pancreatic digest of gelatin ......................................................... 14.5g

Brain heart, solids from infusion .................................................. 6.0g

Peptic digest of animal tissue........................................................ 6.0g

NaCl .............................................................................................. 5.0g

Casein............................................................................................ 5.0g

Glucose ......................................................................................... 3.0g

Na2HPO4 ....................................................................................... 2.5g

Preparation of Brain Heart Infusion Broth: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Solution A: Add 8.0g of ionagar to 440.0mL of brain

heart infusion broth. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Spirolate Broth:

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Glucose ......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 2.5g

L-Cysteine·HCl·H2O...................................................................... 1.0g

Sodium thioglycolate .................................................................... 0.5g

Palmitic acid ............................................................................... 0.05g

Stearic acid.................................................................................. 0.05g

Oleic acid .................................................................................... 0.05g

Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Broth: Add components to distilled/de

ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 25°.

Mucin Solution:

Composition per 20.0mL:

Mucin ............................................................................................ 0.2g

Preparation of Mucin Solution: Add mucin to distilled/deionized

water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine 440.0mL of solu

tion A, 440.0mL of spirolate broth, 100.0mL of rabbit serum, and

20.0mL of mucin solution. Mix thoroughly. Aseptically distribute into

sterile tubes or flasks.

Use: For the isolation of intestinal treponemes.

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Treponema Medium