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培養(yǎng)基配方
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Treponema Medium
[所屬分類(lèi):培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Treponema Medium
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per liter:
Cysteine·HCl·H2O ........................................................................ 1.0g
Glucose ......................................................................................... 1.0g
Nicotinamide................................................................................. 0.4g
Spermidine·4HCl ........................................................................ 0.15g
Sodium isobutyrate ..................................................................... 0.02g
Thiamine pyrophosphate ........................................................... 5.0mg
PPLO broth ............................................................................900.0mL
Rabbit serum, inactivated ......................................................100.0mL
pH 7.8 ± 0.2 at 25°C
PPLO Broth:
Composition per 900.0mL:
Beef heart, infusion from solids.................................................. 50.0g
Peptone ....................................................................................... 10.0g
NaCl.............................................................................................. 5.0g
Preparation of PPLO Broth: Add components to distilled/deion
ized water and bring volume to 900.0mL. Mix thoroughly.
Preparation of Medium: Combine components, except rabbit se
rum. Mix thoroughly. Filter sterilize. Aseptically add sterile rabbit se
rum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of oral treponemes. For the cultivation of
Treponema denticola, Treponema macrodentium, and Treponema ora
lis.
