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培養(yǎng)基配方
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Treponema Medium
[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Treponema Medium
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per liter:
Heart infusion broth, modified...............................................450.0mL
Spirolate broth........................................................................450.0mL
Rabbit serum, inactivated ......................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Heart Infusion Broth, Modified:
Composition per liter:
Beef heart, solids from infusion................................................ 500.0g
Tryptose ...................................................................................... 10.0g
NaCl .............................................................................................. 5.0g
Asparagine .................................................................................... 2.5g
Sodium thioglycolate.................................................................... 2.5g
Pancreatic digest of casein............................................................ 2.5g
Preparation of Heart Infusion Broth, Modified: Add compo
nents to distilled/deionized water and bring volume to 1.0L. Mix thor
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C.
Spirolate Broth:
Composition per liter:
Pancreatic digest of casein.......................................................... 15.0g
Glucose ......................................................................................... 5.0g
Yeast extract.................................................................................. 5.0g
NaCl.............................................................................................. 2.5g
L-Cysteine·HCl·H2O...................................................................... 1.0g
Sodium thioglycolate.................................................................... 0.5g
Palmitic acid ............................................................................... 0.05g
Stearic acid.................................................................................. 0.05g
Oleic acid.................................................................................... 0.05g
Linoleic acid ............................................................................... 0.05g
Preparation of Spirolate Broth: Add components to distilled/de
ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Aseptically combine 450.0mL of cooled,
sterile spirolate broth, 450.0mL of cooled, sterile heart infusion broth,
modified, and 100.0mL of rabbit serum. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation of treponemes.
