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培養(yǎng)基配方
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Treponema Medium
[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-18] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Treponema Medium
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jlkcpj.cn
Composition per 500.0mL:
Beef heart, solids from infusion................................................ 250.0g
Sucrose........................................................................................ 50.0g
Tryptose ........................................................................................ 5.0g
NaCl.............................................................................................. 2.5g
Yeast extract.................................................................................. 2.5g
Agar .............................................................................................. 0.5g
Sodium thioglycolate.................................................................. 0.38g
MgSO4 ........................................................................................ 0.05g
Horse serum, inactivated .......................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 400.0mL. Mix thorough
ly. Adjust pH to 7.4. Gently heat and bring to boiling. Distribute into
tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Prior to inoculation, add 1.0mL sterile horse se
rum to each tube.
Use: For the cultivation and maintenance of Treponema pallidum and
other Treponema species.
